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赤霉素与盐碱互作条件及种子形态性状对瘤囊薹草种子萌发的影响

Effects of seed morphological traits on Carex schmidtii germination under gibberellin and saline-alkali interactions

  • 摘要: 瘤囊薹草(Carex schmidtii)是东北地区典型的湿地植物,在维持湿地生态系统水文稳定、碳汇功能及生物多样性等方面具有重要生态作用。然而,其种子天然萌发率极低,严重限制了大面积开展基于种子撒播萌发的湿地植被恢复与生态修复应用。为了明确环境因子与种子形态性状对瘤囊薹草种子萌发的综合影响,以瘤囊薹草种子为研究对象,测定其种子形态性状(长度、宽度、投影面积、质量),并采用不同浓度的GA3(0.05~0.20 g/L)浸种24 h,在0~50 mmol/L的盐碱梯度条件下,开展种子萌发实验。研究结果表明,盐碱胁迫显著抑制瘤囊薹草种子的萌发速率和发芽指数,赤霉素的一种GA3处理及其与盐碱胁迫的交互作用显著影响种子的初始萌发天数;在种子形态性状中,Spearman相关分析表明,瘤囊薹草种子质量是最主要的影响因子,与萌发率、萌发速率、发芽指数、发芽势及发芽峰值显著正相关,并与初始萌发天数呈负相关,种子长度、宽度和投影面积与所有萌发指标无显著关系;综合回归分析结果表明,在本实验条件下瘤囊薹草的内在种子形态性状对萌发过程的影响总体高于外界环境因素,质量较大的种子在一定程度上可缓解盐碱胁迫的抑制效应。研究探讨了基于种子形态性状的种子预筛选及最优环境条件和激素处理方案,可为提升典型湿地植物瘤囊薹草种子的萌发率提供数据支撑与依据。

     

    Abstract: Carex schmidtii, a typical wetland plant species in Northeast China, plays a crucial ecological role in maintaining wetland ecosystem stability, carbon sequestration capacity and biodiversity. However, its extreme low seed germination percentage severely restricts the large-scale application of seed-based wetland vegetation restoration and ecological restoration practices. To clarify the combined regulatory effects of environmental factors and seed morphological traits on seed germination, this study measured seed morphological traits of C. schmidtii (length, width, projected area, and weight) and conducted germination experiments across gibberellin solution gradients (0.05-0.20 g/L) and saline-alkali gradients (0-50 mmol/L). The results showed that saline-alkali stress significantly inhibited the germination rate and germination index of C. schmidtii seeds, while gibberellin treatment and its interaction with saline-alkali stress significantly affected the days to first germination. In the Spearman correlation analysis, among seed morphological traits, seed weight was the most influential factor, showing significant positive correlations with germination percentage, germination rate, germination index, germination potential and peak value, and a negative correlation with days to first germination. In contrast, seed length, width and projected area had no significant relationship with any germination parameter. Integrated regression analyses further indicated that, under the experimental conditions of this study, intrinsic seed morphological traits exerted stronger effects on the seed germination process than external environmental factors, with heavier seeds partially mitigating the inhibitory effects of saline-alkali stress. These results provide a scientific basis for the selection and pretreatment of C. schmidtii seeds based on morphological traits in wetland restoration practices.

     

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