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果囊、温度和不同试剂对臌囊薹草种子萌发的影响

Effects of utricle, temperature and different reagents on seed germination of Carex schmidtii

  • 摘要: 为了解决臌囊薹草(Carex schmidtii)种子萌发率低的问题,开展了温度、果囊以及NaOH、赤霉素(Gibberellin A3,GA3)和二者混合处理对种子萌发的影响研究。研究结果表明,种子在恒温环境下无法萌发,而在变温环境下能够萌发;在25 ℃/15 ℃(白天25 ℃,夜晚15 ℃)环境下,去除果囊显著提高了种子的萌发率和萌发速率;在相同环境下,GA3和NaOH均显著提高了无果囊种子的萌发率和萌发速率,缩短了初次萌发时间,破除了种子的休眠;NaOH处理的促进效果较GA3处理更佳,但高浓度的NaOH溶液容易腐蚀种子,二者混合处理效果最好(p<0.05);最佳处理条件为,将无果囊种子置于1% NaOH溶液中浸泡2 h,然后置于0.2 g/L GA3溶液中浸泡2 h,并处于25 ℃/15 ℃的环境中萌发,20 d萌发率可达到51.67%。本研究初步揭示了臌囊薹草种子的萌发特性,发现了打破种子休眠的方法,为退化湿地恢复提供了理论和技术支撑。

     

    Abstract: To solve the problem of low germination rate of Carex schmidtii seeds, we conducted research on the effects of temperature, utricle, NaOH, Gibberellins A3 (GA3) and their mixed treatments on seed germination. The results showed that seeds could not germinate under a constant temperature environment, but could germinate under a variable temperature environment. Under 25℃/15℃ (25℃ during the day and 15℃ at night), removing the utricles significantly improved the germination rate and germination rate of seeds. Under the same environment, both GA3 and NaOH treatments significantly improved the germination rate of seed without utricles, shortened the initial germination time, and broke the dormancy of seeds. The promotion effect of NaOH treatment was better than that of GA3 treatment, but high concentration of NaOH solution was prone to corroding seeds, and the mixture of NaOH and GA3 was the best (p<0.05). The optimal treatment conditions were as follows: the seeds without utricle were soaked in 1% NaOH for 2 h, then soaked in 0.2 g/L GA3 for 2 h, and germinated in an environment of 25℃/15℃. The seed germination rate could reach 51.67% after 20 days. This study preliminarily revealed the germination characteristics of C. schmidtii seeds, discovered methods to break seed dormancy, and provided theoretical and technical support for the restoration of degenerated wetlands.

     

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